Regulatory

Part:BBa_J100231

Designed by: Kelly Friers   Group: Campbell M Lab   (2015-09-10)


tolC Constitutive Promoter in E. coli

This promoter sequence is 60 base paris long. The original sequence was 58 base pairs long without the sticky ends, so we shortened the sequence to 56 base pairs (taking 2 base pairs off from the 5' end) and we added the appropriate sticky ends to each side. The -10 sequence is TTAAAT and the -35 sequence is TTGACT. it is believed to be a constitutive promoter. We will be using it to promote RFP expression in E. coli. We are experimenting with the p1 promoter to tolC (which is constitutive) rather than the p2 promoter for tolC, which requires chemicals to be activated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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